生物素3 末端DNA標記試劑盒
Cat No | BIOK2010 |
Conjugate | |
Type | Kit |
Source | N/A |
Size | 20次 |
Application | NB; SB; EMSA; colony hybridization |
Format | Liquid |
Concentration | Please refer to the vial lable for the specific concentration. |
Buffer | Supplied in PBS. |
Storage | Store at -20 degree. Avoid repeated freeze/thaw cycles. |
Synonyms | Biotin 3 End DNA Labeling Kit;生物素3 末端DNA標記kit |
Purification | |
Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
MolecularWeight | |
Description | Terminal Deoxynucleotidyl Transferase(TdT)可以在不依賴于模板的情況下催化在DNA的3 -OH端加上dNTP的反應。關于TdT催化雙鏈DNA末端加dNTP的反應效率,3 端突出的雙鏈DNA要比平端或3 端縮進的雙鏈DNA高很多。TdT催化單鏈DNA末端加dNTP的反應效率要比雙鏈DNA高很多。在適當?shù)臈l件下,TdT也可以催化RNA 3 末端加NTP的反應。 |
Background | 生物素3 末端DNA標記試劑盒(Biotin 3 End DNA Labeling Kit)是一種通過Terminal Deoxynucleotidyl Transferase(TdT)把生物素標記的dUTP添加到DNA 3 末端的試劑盒。通常DNA的3 末端被標記后不會干擾雜交反應,也不會干擾基于序列特異性蛋白結合的EMSA檢測;因此生物素標記的DNA探針可以用于常規(guī)的Northern、Southern、EMSA(即gel shift)以及colony hybridization或原位雜交等。 |