Cat No | BIOK2014 |
Conjugate | |
Type | Kit |
Source | N/A |
Size | 20次 |
Application | EMSA |
Format | Liquid |
Concentration | Please refer to the vial lable for the specific concentration. |
Buffer | Supplied in PBS. |
Storage | Store at -20 degree. Avoid repeated freeze/thaw cycles. |
Synonyms | EMSA探針生物素標(biāo)記試劑盒;EMSA Probe Biotin Labeling Kit |
Purification | |
Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
MolecularWeight | |
Description | Terminal Deoxynucleotidyl Transferase(TdT)可以在不依賴(lài)于模板的情況下催化在DNA的3 -OH端加上dNTP的反應(yīng)。關(guān)于TdT催化雙鏈DNA末端加dNTP的反應(yīng)效率,3 端突出的雙鏈DNA要比平端或3 端縮進(jìn)的雙鏈DNA高很多。TdT催化單鏈DNA末端加dNTP的反應(yīng)效率要比雙鏈DNA高很多。在適當(dāng)?shù)臈l件下,TdT也可以催化RNA 3 末端加NTP的反應(yīng)。 |
Background | EMSA探針生物素標(biāo)記試劑盒(EMSA Probe Biotin Labeling Kit)是一種通過(guò)Terminal Deoxynucleotidyl Transferase(TdT)把生物素標(biāo)記的dUTP添加到單鏈DNA 3 末端,然后通過(guò)退火產(chǎn)生生物素標(biāo)記的EMSA探針的試劑盒。通常DNA的3 末端被標(biāo)記后不會(huì)干擾基于序列特異性蛋白結(jié)合的EMSA檢測(cè)。 |