Cat No | BLYK-002 |
Conjugate | |
Clone | 其他試劑盒 |
Size | N/A |
Application | N/A |
ApplicationNote | Fragmentation |
Purity | Liquid |
Buffer | Please refer to the vial lable for the specific concentration. |
Preservative | Supplied in PBS. |
Storage | |
Synonyms | The F(ab′)2 Preparation Kit enable sefficient generation of F(ab′)2from IgG. This kit uses Immobilized Pepsin, a nonspecific endopeptidase that is active only at acid pH and irreversibly denatured at neutral or alkaline pH. Pepsin digestion typically produces a F(ab′)2fragment (~110kDa by SDS-PAGE under non-reducing conditions)and numerous small peptides of the Fc portion (Figure 1). The resulting F(ab′)2fragment is composed of a pair of Fab′ units connected by two disulfide bonds. The Fc fragment is extensively degraded and can be separated from F(ab′) 2 by dialysis, gel filtration or ion exchange chromatography. |
Purification | |
Note | Upon receipt store at 4-8°C |
MolecularWeight | Protein A |
Description | F(ab′)2 Micro Preparation Kit |
Background | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |