Cat No | BIOK2016 |
Conjugate | |
Type | 其他試劑盒 |
Source | N/A |
Size | 20次 |
Application | DNA Blunting |
Format | Liquid |
Concentration | Please refer to the vial lable for the specific concentration. |
Buffer | Supplied in 335mM Tris-HCl(pH8.8 at 25℃), 33mM MgCl2, 5mM DTT, 84mM(NH4)2SO4. |
Storage | Store at -20 degree. Avoid repeated freeze/thaw cycles. |
Synonyms | DNA末端平滑試劑;DNA Blunting Kit |
Purification | |
Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
MolecularWeight | |
Description | 經(jīng)過本試劑盒處理產(chǎn)生的平末端DNA片段可以用于常規(guī)的平端連接反應(yīng)。粘末端有兩種,一種為5 端突出末端,另外一種為3 端突出末端。本試劑盒可以將5 端突出末端補(bǔ)平,也可以將3 端突出末端削平(也稱打平)。當(dāng)末端為5 端突出末端時(shí),T4 DNA Polymerase可以利用其5 →3 DNA聚合酶活性將末端補(bǔ)平;當(dāng)末端為3 端突出末端時(shí),T4 DNA Polymerase可以利用其3 →5 DNA外切酶活性將末端削平。 |
Background | DNA末端平滑試劑盒(DNA Blunting Kit)是利用T4 DNA聚合酶(T4 DNA Polymerase)的5 →3 DNA聚合酶活性和3 →5 的DNA外切酶活性,用于將酶切或PCR等反應(yīng)后產(chǎn)生的粘末端轉(zhuǎn)變?yōu)槠侥┒说脑噭┖小?/td> |