Membrane and Cytosol Protein Extraction Kit
Cat No | BIOS-2029K |
Conjugate | |
Type | 其他試劑盒 |
Size | 50T; 100T |
Application | Extraction |
Format | Liquid |
Storage | Store at -20 degree |
Synonyms | Membrane and Cytosol;Membrane and Cytosol Protein Extraction Kit;蛋白提取試劑盒 |
Purification | |
MolecularWeight | |
Description | |
Background | The cell membrane/cytoplasmic protein extraction kit is used to quickly and conveniently separate cell/tissue membrane and cytoplasmic proteins. The extracted membrane proteins include not only proteins on the plasma membrane, but also membrane proteins on the mitochondrial membrane, endoplasmic reticulum membrane, Golgi body membrane, etc. Main working principle: moderately crush the cells through homogenization, remove the precipitation generated by the nucleus and a few unbroken cells through low-speed centrifugation, then take the supernatant for high-speed centrifugation to obtain the cell membrane precipitation and the supernatant containing cytoplasmic protein, and then extract the membrane protein from the precipitation through the optimized membrane protein extraction reagent. The whole process only takes about 90 minutes to complete. The extracted protein can be used in subsequent experiments such as SDS-PAGE, Western and enzyme activity determination. |
We can provide a variety of products such as diagnostic proteins; target proteins; cytokines; etc.