Cat No | BSB-015 |
Conjugate | |
Type | 膜再生 |
Source | N/A |
Size | 500ml |
Application | WB |
Format | Liquid |
Storage | Store at RT. |
Synonyms | Stripping buffer |
Purification | |
Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
MolecularWeight | |
Description | 使用膜再生液可以對同一張膜進行多次 Western Blot 檢測. 在室溫條件下, 將用過的膜浸泡在膜再生液 10-60 分鐘, 可在不影響抗原蛋白的情況下清除膜上結合的抗體. 隨后, 可以使用不同的抗體進行下一輪 Western Blot 實驗. 適宜從少量樣品多次檢測不同蛋白質. 即使樣品量并不匱乏, 采用這一策略多次檢測同一 張膜上的蛋白, 能省去給藥處理, 蛋白電泳和膜轉移等耗費性步驟. 而且可以消除重新上樣而帶來的誤差, 使可比性更強. |
Background | Western Blot Stripping Buffer is a gentle method for breaking antibody-antigen interactions to allow nitrocellulose and PVDF membranes to be reprobed several times using different antibodies, saving time and conserving samples. Ideal for use with chemiluminescent substrates. |