Cat No | BSC-001 |
Conjugate | |
Type | 轉(zhuǎn)膜效率檢測(cè) |
Source | N/A |
Size | 100ml |
Application | WB |
Format | Liquid |
Storage | Store at RT. |
Synonyms | Ponceau S |
Purification | |
Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
MolecularWeight | |
Description | 使用說(shuō)明:1.此產(chǎn)品為10×保存液, 使用前用ddH2O稀釋10倍配制成工作液. 2.取麗春紅染色液3-10ml, 染色液的用量, 依膜的大小而定. 2.將PVDF膜, 硝酸纖維素膜或醋酸纖維素膜浸沒(méi)在麗春紅染色液中, 搖動(dòng)5-10分鐘或更長(zhǎng)時(shí)間. 3.取出印跡膜, 用蒸餾水, PBS或其它適當(dāng)溶液漂洗2-3次, 可出現(xiàn)清晰條帶, 記錄結(jié)果. 4.用蒸餾水, PBS或其它適當(dāng)溶液漂洗2-3次, 每次3-5分鐘, 去除麗春紅, 進(jìn)行后續(xù)的WB檢測(cè). |
Background | Ponceaus S Staining Solution is a ready-to-use membrane stain for evaluating the transfer efficiency of a western blot. This stain is compatible with nitrocellulose and PVDF membranes. |