Cat No | BSK-009 |
Conjugate | |
Type | 顯影 |
Source | N/A |
Size | 500ml |
Application | WB |
Storage | Store at 2-8℃. Protect from light for one year. |
Synonyms | Hypersensitivity ECL Chemiluminescence Kit |
Purification | |
Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
MolecularWeight | |
Description | 顯色步驟 1, 執(zhí)行常規(guī)SDS-PAGE, 轉(zhuǎn)膜和Western Blot封閉, 一抗孵育, 二抗孵育操作. 2, 二抗適用HRP標(biāo)記二抗, 適用恰當(dāng)?shù)南♂尡壤? 3, 制備ECL工作液:A液和B液等體積的混合, 例如1ml A液和1ml B液混合, 混勻后即可使用. 用量以充分覆蓋印跡膜為基準(zhǔn), 每10 cm2膜需要大約1 ml工作液. 4, 將印跡膜有蛋白的一面朝上平整的鋪在一張平板上, 加上配好的發(fā)光底物工作液. 5, 將工作液均勻滴加在印跡膜上, 孵育1-5min. 可將一潔凈的保鮮膜或透明的玻璃紙平整的鋪在孵育有工作液的印跡膜上, 輕輕趕出其間的氣泡. 6, 在暗室中取一張X膠片小心置于膜上, 曝光5秒至1分鐘, 立即顯影定影. 7, 根據(jù)其發(fā)光的強(qiáng)度, 縮短或延長(zhǎng)X片的曝光時(shí)間(對(duì)微弱信號(hào), 曝光時(shí)間可延長(zhǎng)至數(shù)小時(shí)), 或者曝光一系列不同的時(shí)間后再顯影定影挑選一張滿意的. 也可用合適的照相器材直接記錄蛋白膜的化學(xué)發(fā)光圖像. 安全性: 無特殊毒性, 按普通化學(xué)品處理. 如果不慎與眼, 皮膚和衣物接觸, 請(qǐng)立刻用大量清水沖洗. |
Background | The product has stable detection results and high sensitivity, which can greatly save valuable samples as well as the amount of primary and secondary antibodies. |