Cat No | BST-008 |
Conjugate | |
Type | 固定液 |
Source | N/A |
Size | 50mL |
Application | IHC, FCM |
Format | Liquid |
Storage | Store at 2-8℃. |
Synonyms | Fixed fluid (flow cytometry);flow cytometry Fixative solution;Fixation Buffer(flow cytometry);Fixative solution (FCM);固定液(流式細(xì)胞);流式細(xì)胞固定液; |
Purification | |
Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
MolecularWeight | |
Description | 實(shí)驗(yàn)方法: 1. 收獲待測(cè)細(xì)胞, 1000 rpm離心10分鐘, 棄上清. 2. 加入500ul預(yù)冷 ( 4℃) 固定液. 3. 室溫孵育10分鐘. 4. 1000 rpm離心10分鐘. 5. 棄上清, 加入PBS洗一次, 1000rpm離心10分鐘. 6. 棄上清, 加入1.5 ml破膜劑. 7. 室溫孵育3-5 分鐘. 8. 1000 rpm離心5 分鐘. 9. 棄上清, 加入適量細(xì)胞洗液重懸細(xì)胞. 10. 加入檢測(cè)抗體進(jìn)行染色后, 用流式細(xì)胞儀進(jìn)行分析. |
Background | Fixed fluid and membrane-breaking agent are used for cell membrane treatment before intracellular cytokine staining. |