Cat No | BSW-001 |
Conjugate | |
Type | 洗滌液 |
Source | N/A |
Size | 60ml |
Application | WB |
Format | Liquid |
Storage | Store at 2-8℃ for one year. |
Synonyms | WB washing solution 10× |
Purification | |
Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
MolecularWeight | |
Description | 使用說明:取適量WB洗滌液, 用去離子水10倍稀釋. 在一抗或二抗孵育結(jié)束后, 加入10ml的WB洗滌液, 使之能蓋住蛋白膜, 在搖床上緩慢搖動(dòng)洗滌3-5分鐘后, 吸盡洗滌液, 再加入新的WB洗滌液. 共洗滌3次, 每次3-5分鐘. 然后進(jìn)行后續(xù)的操作. 如果按照上述洗滌條件, WB的染色結(jié)果背景仍然較高, 可以適當(dāng)延長(zhǎng)洗滌時(shí)間, 并增加洗滌次數(shù). 通常, 延長(zhǎng)洗滌時(shí)間或增加洗滌次數(shù)不會(huì)對(duì)WB的結(jié)果產(chǎn)生負(fù)面影響. |
Background | The product is a washing buffer for peroxidase conjugates in Western blotting. |