Inclusion Body Solubilization and Refolding Kit
Cat No | BLY-005K |
Conjugate | |
Type | 試劑盒 |
Size | 100次 |
Application | |
Concentration | Please refer to the vial lable for the specific concentration. |
Storage | |
Synonyms | Inclusion Body Solubilization and Refolding;Solubilization;Refolding;Inclusion Body |
Purification | |
MolecularWeight | |
Description | |
Background | Inclusion bodies refer to the aggregation of proteins expressed by bacteria within cells, forming inactive solid particles. Generally, it contains more than 50% recombinant protein, with the rest consisting of ribosome components, plasmid DNA, lipopolysaccharides, etc., with a size of 0.5-1um, difficult to dissolve in water, only soluble in denaturing agents such as urea, guanidine hydrochloride, etc. Inclusion bodies can prevent the degradation of expressed proteins by proteases, and their formation reduces the concentration of intracellular expressed proteins, which is beneficial for increasing expression levels. Recombinant proteins obtained through host expression in Escherichia coli often form inclusion bodies, in which the recombinant proteins have only completed partial folding and have not yet formed the correct spatial structure, so they have no biological activity. To obtain active recombinant proteins, subsequent dissolution and refolding (refolding) treatments are required. The most commonly used method for dissolving inclusion bodies is to use strong denaturing agents such as urea, Sarkozy, and guanidine hydrochloride. However, the disadvantage is that the recombinant proteins in the inclusion bodies are completely denatured, which reduces the efficiency of subsequent protein refolding processes. |
We can offer labeled proteins/antibodies using a broad range of intensely fluorescent dyes and labels including FITC; Biotin; Alexa Fluor; Rhodamine B; Cy; Co-Au.