Cat No | BSQ-006 |
Conjugate | |
Type | Protein extraction and quantification |
Source | N/A |
Size | 1ml×10 |
Application | Inhib |
Format | Liquid |
Concentration | Please refer to the vial lable for the specific concentration. |
Storage | Store at -20℃ stable for at least 12 months. |
Synonyms | Protease inhibitor cocktail |
Purification | |
Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
MolecularWeight | |
Description | 哺乳動物細胞或組織提取物中含有許多內(nèi)源性的蛋白酶, 磷酸酶等, 容易導致提取物中的蛋白降解或去修飾, 從而影響后續(xù)的蛋白檢測. 因此在提取物中添加適當?shù)牡鞍酌? 磷酸酶等抑制劑是防止蛋白降解和去修飾的有效方法. Bioss生產(chǎn)的蛋白酶抑制劑混合物(哺乳動物樣品抽提用, 100×) (Protease inhibitor cocktail for mammalian cell and tissue extracts, 100×) 是一種用于哺乳動物細胞或組織蛋白提取的蛋白酶抑制劑混合物, 主要成分有:105mM AEBSF, 80uM Aprotinin, 5mM Bestatin, 1.5mM E64, 2mM Leupeptin and 1.5mM Pepstatin A in DMSO, 并提供一支獨立包裝的0.5M EDTA, pH 8.0, 以便選擇性使用. 注:如果所提取的蛋白質(zhì)用于檢測金屬蛋白酶活性, 則不宜添加EDTA. 本產(chǎn)品為100×濃縮液, 使用時請按1:100的比例加入裂解液中, 用于哺乳動物細胞或組織蛋白的提取, 可有效抑制蛋白降解. 該產(chǎn)品可以抑制絕大多數(shù)蛋白酶活性, 包括絲氨酸蛋白酶, 半胱胺酸蛋白酶, 胃蛋白酶, 胰蛋白酶, 木瓜蛋白酶等. 所提取的蛋白質(zhì)可以用于 Western Blot, 免疫共沉淀等試驗. |
Background | They can inhibit the activity of trypsin, papain, chymotrypsin, elastase, pepsin, metalloproteinase and other proteases, thus effectively inhibiting the degradation of protein, and maintaining the original protein structure. |